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Protocols in Biotechnology
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- 1. Computer Aid Design of Primer, Probe, Gene Fragment
- 2. Gene Construction Using Oligonucleotides
- 3. Retroviral, Vectors
- 4. Site-Directed Mutagenesis
- 5. mRNA Differential Display
- 6. Gene Isolation, Cloning
- 7. DNA Sequencing (GenomxLR, ABI 370A etc.)
- 8. Nucleus Chromosomes Analysis
- 9. Southern, Northern (MD-SF130 Phosphorimager etc.) Hybridzation
- 10. RNA interfence, Gene Silencing/Article/
Data
- 11. Signal Transduction & Molecules Assay
- 12. Nuclear Running off Experiments
- 13. Ligation Chain Reaction
- 14. Quantitative RT-PCR (Lightcycler, Roche),
Long PCR
- 15. Embryo,
Cell Culture, Mutagenesis
- 16. Gene Recombination, Transgenesis (Amaxa, BTX Electorporation
etc.)
- 17. Stable Protein Expression in Mammalian Cells
- 18. Protein Immunoprecipitation, Western Blotting
- 19. Peptide Synthesis ( Protein Technologies-PS3 etc.)
- 20. cDNA Micro-Array Protocol/Forum (GenePix
4000B, Axon)
- 21. Biotechnology of Molecular Genetics
. Pattern Genetics
Signal Transduction and Gene Expression in Cell Fate Determination
Biosystem
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